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The largemouth bass is an important species, and its culture has risen sharply with the surge in fish aquaculture in China. Due to the lack of selective breeding technology for the largemouth bass, the growth rate and disease resistance are low, its sexual maturation is slow, and other serious problems are contributing to a sharp decline in the safety and quality of largemouth bass products in recent decades. Therefore, comprehensive breeding programs to improve the economic performance and promote the modern industrial development of largemouth bass must be considered a priority. Here, a total of adult largemouth bass, including two parents and progenies, were selected to produce the genetic mapping family. Then, a high-density linkage map was constructed based on restriction site—associated DNA sequencing using 6, single-nucleotide polymorphisms SNPs located in 24 linkage groups LGs. The total genetic length of the linkage map was 1,
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